LOCUS       40924_36953       11502 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRGK 366, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11502)
  AUTHORS   Engineer CB, Kranz RG.
  TITLE     Reciprocal leaf and root expression of AtAmt1.1 and root 
            architectural changes in response to nitrogen starvation
  JOURNAL   Plant Physiol. 143 (1), 236-250 (2007)
  PUBMED    17085512
REFERENCE   2  (bases 1 to 11502)
  AUTHORS   Engineer CB, Kranz RG.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2006) Biology, Washington University in St. Louis,
            1 Brookings Drive, # 1137, St. Louis, MO 63130, USA
REFERENCE   3  (bases 1 to 11502)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11502)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2007"; volume: "143"; issue: "1"; pages: "236-250"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUN-2006) Biology, Washington University in St. Louis, 1 
            Brookings Drive, # 1137, St. Louis, MO 63130, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(292..316)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     587..603
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(700..716)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(724..740)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(748..778)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        790..836
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             937..1377
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     CDS             1381..1614
                     /label=VP16 AD
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et 
                     al., 1989)"
     terminator      1621..1873
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      1882..2283
                     /label=full 35S CaMV promoter
                     /note="full 35S CaMV promoter"
                     /regulatory_class="promoter"
     promoter        2121..2167
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             2280..2828
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     misc_feature    2875..3247
                     /label=7S UTR
                     /note="7S UTR"
     protein_bind    3293..3387
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     regulatory      3396..3492
                     /label=minimal 35S CaMV promoter element
                     /note="minimal 35S CaMV promoter element"
                     /regulatory_class="promoter"
     promoter        3448..3494
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             3532..5181
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    5227..6186
                     /label=TML cassette
                     /note="TML cassette"
     protein_bind    6213..6483
                     /label=tetracycline response element
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     promoter        6524..6570
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    complement(6571..6595)
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     rep_origin      complement(6761..7474)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     CDS             8742..8930
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    9035..9175
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9361..9949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     gene            10522..11311
                     /gene="spc/str"
                     /label=spc/str
     CDS             10522..11310
                     /codon_start=1
                     /gene="spc/str"
                     /product="spectinomycin/streptomycin resistance"
                     /label=spc/str
                     /protein_id="ABG75722.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgaggatttt tcggcgctgc gctacgtccg caccgcgttg agggatcaag ccacagcagc
       61 ccactcgacc tctagccgac ccagacgagc caagggatct ttttggaatg ctgctccgtc
      121 gtcaggcttt ccgacgtttg ggtggttgaa cagaagtcat tatcgtacgg aatgccaagc
      181 actcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt
      241 ttcacgccct tttaaatatc cgttattcta ataaacgctc ttttctctta ggtttacccg
      301 ccaatatatc ctgtcaaaca ctgatagttt tatcggtgtc ggggctggct taactatgcg
      361 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
      421 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
      481 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
      541 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
      601 agtgaattcg agctcggtac ccgggggcgc gccggatcct taattaagtc tagagtcgac
      661 tgtttaaacc tgcaggcatg caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
      721 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtcaaga
      781 taaaccttcg caagaccctt cctctatata aggaagttca tttcatttgg agaggacaca
      841 gaaaaatttg ctacattgtt tcacaaactt caaatattat tcatttattt gtcagctttc
      901 aaactctttg tttcttgttt gttgattaga tctaccatga agctcctgtc ctccatcgag
      961 caggcctgcg acatctgccg cctcaagaag ctcaagtgct ccaagaagaa gccgaagtgc
     1021 gccaagtgtc tgaagaacaa ctgggagtgt cgctactctc ccaaaaccaa gcgctccccg
     1081 ctgacccgcg cccacctcac cgaagtggag tcccgcctgg agcgcctgga gcagctcttc
     1141 ctcctgatct tccctcgaga ggacctcgac atgatcctga aaatggactc cctccaggac
     1201 atcaaagccc tgctcaccgg cctcttcgtc caggacaacg tgaacaaaga cgccgtcacc
     1261 gaccgcctgg cctccgtgga gaccgacatg cccctcaccc tgcgccagca ccgcatcagc
     1321 gcgacctcct cctcggagga gagcagcaac aagggccagc gccagttgac cgtctcgacg
     1381 gcccccccga ccgacgtcag cctgggggac gagctccact tagacggcga ggacgtggcg
     1441 atggcgcatg ccgacgcgct agacgatttc gatctggaca tgttggggga cggggattcc
     1501 ccggggccgg gatttacccc ccacgactcc gccccctacg gcgctctgga tacggccgac
     1561 ttcgagtttg agcagatgtt taccgatgcc cttggaattg acgagtacgg tgggtagccc
     1621 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
     1681 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     1741 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
     1801 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
     1861 atgttactag atccctaggc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt
     1921 ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc
     1981 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
     2041 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
     2101 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
     2161 gagaggacac gctgaaatca ccagtctctc tctacaaatc tatctctctc tattttctcc
     2221 ataataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca
     2281 tgagcccaga acgacgcccg gccgacatcc gccgtgccac cgaggcggac atgccggcgg
     2341 tctgcaccat cgtcaaccac tacatcgaga caagcacggt caacttccgt accgagccgc
     2401 aggaaccgca ggagtggacg gacgacctcg tccgtctgcg ggagcgctat ccctggctcg
     2461 tcgccgaggt ggacggcgag gtcgccggca tcgcctacgc gggcccctgg aaggcacgca
     2521 acgcctacga ctggacggcc gagtcaaccg tgtacgtctc cccccgccac cagcggacgg
     2581 gactgggctc cacgctctac acccacctgc tgaagtccct ggaggcacag ggcttcaaga
     2641 gcgtggttgc tgtcatcggg ctgcccaacg acccgagcgt gcgcatgcac gaggcgctcg
     2701 gatatgcccc ccgcggcatg ctgcgggcgg ccggcttcaa gcacgggaac tggcatgacg
     2761 tgggtttctg gcagctggac ttcagcctgc cagtaccgcc ccgtccggtc ctgcccgtca
     2821 ccgagatttg accgtccttt gtcttcaatt ttgagggctt tttactgaat aagtatgtag
     2881 tactaaaatg tatgctgtaa tagctcatag tgagcgagga aagtatcggg ctatttaact
     2941 atgacttgag ctccatctat gaataaataa atcagcatat gatgcttttg ttttgtgtac
     3001 ttcaactgtc tgcttagcta atttgatatg gttggcactt ggcacgtata aatatgctga
     3061 agtaatttac tctgaagcta aattaactag attagatgag tgtattatat acaaaaggca
     3121 ttaaatcaga tacatcttag acaaattgtc acggtctacc agaaaagaaa ttgcatttgt
     3181 ttttgggtct ttcagactga caagatcgat ctgaagtcta aacaattcta agaggtatca
     3241 tgtagcaatg tcctgccaca atattgaacg cgtagcttgc atgcctgcag gtcggagtac
     3301 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt
     3361 cctccgagcg gagtactgtc ctccgagcgg agactctaga agctactcca cgtccataag
     3421 ggacacatca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
     3481 tcatttggag aggacgacct gcaggtcgac ggatctccta ggaagctttc catggaagac
     3541 gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga
     3601 gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca
     3661 gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg
     3721 gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa
     3781 aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg
     3841 cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct
     3901 accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc
     3961 ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg
     4021 atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca
     4081 gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt
     4141 ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga
     4201 gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc
     4261 catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc
     4321 ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa
     4381 agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa
     4441 tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc
     4501 ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact
     4561 gagactacat cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt
     4621 aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc
     4681 gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac
     4741 aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata
     4801 gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag
     4861 tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac
     4921 atcttcgacg caggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc
     4981 gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc
     5041 agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg
     5101 aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag
     5161 aagggcggaa agatcgccgt gtaattctag agaattcggg aggaaattac actgaggaag
     5221 gagaagatga cgacgatgag atggacgatg aaggggaggc tggtggagcg gaaccaagag
     5281 agtgtcagat cggaaacctt atcaattatc cgatcattgc tttagggtca tgcgatcttt
     5341 ccgcataatt cccgtcgccg acacctaata aagtcggcta atctatgtga ttgagtgtgt
     5401 cttgactttg ttattttgca tgtttccaat gtcatttagt aacgaaataa acgttatcct
     5461 cttctaaaag caggctgtgt tttcggcaaa catcgccacc catcgctagt ttttctaaaa
     5521 gtgttctaag ctagcctggt aataatctat acgagcttat atttctaatc attgccgaaa
     5581 aatcctgttt cgaaataatt ttgtaactct ctttaatatc accacgatca cacaagaaga
     5641 agaattaaat ataacattta tcaagcccac gatgaacatg gcgaaaatta caagcgaaca
     5701 caattgtctt attcattaat aattaattca acaaagttct cagacactcc ttgatttaac
     5761 agaagatttt ataagcacct ctatgatgca acaaccaaaa cgacatagac ttcgaaatcc
     5821 cgccccataa aaactagata gcaccatcta actccacgtc ttcaagagca gcctccggga
     5881 ggaaagctga acagcccaca gcgatcaaaa gataatccaa tggttggccg gcgcttggca
     5941 ttttcctttt ccaaacgtcc ttgctttgtc caaaagtgtt ccggacgact cctatgcgtt
     6001 cgaactcgtt gctggcctga attgctagaa agtacgagga tgaatattca tttttgctgt
     6061 agttgatccg accaccgtct ccgcttaagg cttgcagttg cgccctcacc tgccggaatg
     6121 tataaccatc agaatagggt tccagtgtcg tggcaagcaa tccatctgaa gcatttgaaa
     6181 actagtcgtt aactgcagct cgagtttacc actccctatc agtgatagag aaaagtgaaa
     6241 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     6301 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     6361 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     6421 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     6481 agaaaagtga aagtcgagct cggtacccgg gtcgagtcta gacgcaagac ccttcctcta
     6541 tataaggaag ttcatttcat ttggagagga atttacaatt gaatatatcc tgccgccgct
     6601 gccgctttgc acccggtgga gcttgcatgt tggtttctac gcagaactga gccggttagg
     6661 cagataattt ccattgagaa ctgagccatg tgcaccttcc ccccaacacg gtgagcgacg
     6721 gggcaacgga gtgatccaca tgggactttt cctagcttgg ctgccatttt tggggtgagg
     6781 ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt agcgggccgg
     6841 gagggttcga gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca
     6901 gccctggtta aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa agacaggtta
     6961 gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc ctgtggacag
     7021 cccctcaaat gtcaataggt gcgcccctca tctgtcagca ctctgcccct caagtgtcaa
     7081 ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc gcagggcact
     7141 tatccccagg cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc
     7201 gatttgcgag gctggccagc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt
     7261 caacgccgcg ccgggtgagt cggcccctca agtgtcaacg tccgcccctc atctgtcagt
     7321 gagggccaag ttttccgcga ggtatccaca acgccggcgg ccggccgcgg tgtctcgcac
     7381 acggcttcga cggcgtttct ggcgcgtttg cagggccata gacggccgcc agcccagcgg
     7441 cgagggcaac cagcccggtg agcgtcggaa agggtcgatc gaccgatgcc cttgagagcc
     7501 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
     7561 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc
     7621 ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga
     7681 atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag
     7741 aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc
     7801 gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg
     7861 atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt
     7921 caaggatcgc tcgcggctct taccagccta acttcgatca ttggaccgct gatcgtcacg
     7981 gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc
     8041 ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc
     8101 tgaatggaag ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc
     8161 aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt
     8221 ccgccatctc cagcagccgc acgcggcgca tctcgggcag cgttgggtcc tggccacggg
     8281 tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg
     8341 gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt
     8401 ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa
     8461 cgcggaagtc agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc
     8521 taccctgtgg aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt
     8581 ttctctggtc ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc
     8641 gggcatgttc atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatca
     8701 ttacccccat gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa
     8761 ccgcccttaa catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca
     8821 acgagctgga cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg
     8881 agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     8941 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     9001 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
     9061 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     9121 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
     9181 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9241 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9301 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9361 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9421 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9481 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9541 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9601 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9661 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9721 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9781 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     9841 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9901 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9961 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    10021 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    10081 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10141 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10201 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10261 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10321 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10381 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg
    10441 tcgggagcac aggatgacgc ctaacaattc attcaagccg acaccgcttc gcggcgcggc
    10501 ttaattcagg agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
    10561 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
    10621 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
    10681 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
    10741 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
    10801 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
    10861 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
    10921 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
    10981 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
    11041 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
    11101 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
    11161 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctaggcaggc ttatcttgga
    11221 caagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgttca ctacgtgaaa
    11281 ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc
    11341 ttcgcggcgc ggcttaactc aagcgttaga tgctgcaggc atcgtggtgt cacgctcgtc
    11401 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    11461 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg at
//
